ZGPAT Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, E |
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Primary Accession | Q8N5A5 |
Other Accession | NP_115916, 133925793 |
Reactivity | Human |
Host | Chicken |
Clonality | Polyclonal |
Isotype | IgY |
Calculated MW | 57359 Da |
Application Notes | ZGPAT antibody can be used for detection of ZGPAT by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 84619 |
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Target/Specificity | ZGPAT; Multiple isoforms of ZGPAT are known to exist. |
Reconstitution & Storage | ZGPAT antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | ZGPAT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZGPAT |
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Synonyms | GPATC6, GPATCH6, KIAA1847, ZC3H9, ZC3HDC |
Function | Transcription repressor that specifically binds the 5'- GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis. |
Cellular Location | Nucleus. |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ZGPAT Antibody: The Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT), is a transcriptional repressor that regulates several cellular pathways including EGFR pathways that are involved in cell proliferation, migration and survival. ZGPAT recruits the Mi-2/NuRD complex and represses the expression of EGFR. ZGPAT is down-regulated in breast carcinomas and its expression is negatively correlated with that of EGFR, suggesting that it may be a potential target for breast cancer therapy. Multiple isoforms of ZGPAT are known to exist, including sZIP, which binds to the Mi-2/NuRD complex but lacks the DNA binding component of full-length ZGPAT, thereby antagonizing transcription repression and growth inhibition by ZGPAT.
References
Li R, Zhang H, Yu W, et al. ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis. EMBO J. 2009; 28:2763-6.
Yu W, Li R, Gui B, et al. sZIP, an alternative splice variant of ZIP, antagonizes transcription repression and growth inhibition by ZIP. J. Biol. Chem. 2010; 285:14301-7.
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