|Application ||WB, IF, E|
|Other Accession||NP_068355, 11277468|
|Calculated MW||18695 Da|
|Application Notes||SIVA antibody can be used for detection of EPAC1 by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||SIVA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIVA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa- B and promotes T-cell receptor-mediated apoptosis.|
|Cellular Location||Cytoplasm. Nucleus. Note=In the nucleus, accumulates in dot-like structures|
|Tissue Location||Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon|
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Provided below are standard protocols that you may find useful for product applications.
SIVA Antibody: SIVA plays an important role in the apoptotic pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. SIVA is a small cysteine-rich protein with a unique N-terminal amphipathic helical region, a death domain homology region (DDHR) and a C-terminal cysteine-rich B-box-like or zinc-finger-like structure. The DDHR domain binds to CD27 and induces apoptosis in T cells through a caspase-dependent mitochondrial pathway. SIVA inhibits BCL2L1 isoform Bcl-xL anti-apoptotic activity and inhibits activation of NFκ-B and promotes T-cell receptor-mediated apoptosis. SIVA represents a potential therapeutic target for the treatment of neuronal cell death diseases.
Prasad KV, Ao Z, Yoon Y, et al. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. USA 1997; 94:6346-51.
Py B, Slomianny C, Auberger P, et al. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immunol. 2004; 172:4008-17.
Resch U, Schichl YM, Winsauer G, et al. Siva1 is a XIAP-interacting protein that balances NFkappaB and JNK signalling to promote apoptosis. J. Cell Sci. 2009; 122:2651-61
Jacobs SB, Basak S, Murray JI, et al. Siva is an apoptosis-selective p53 target gene important for neuronal cell death. Cell Death Differ. 2007; 14:1374-85.
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