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SIVA Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | O15304 |
| Other Accession | NP_068355, 11277468 |
| Reactivity | Human, Mouse |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Calculated MW | 18695 Da |
| Application Notes | SIVA antibody can be used for detection of EPAC1 by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 10572 |
|---|---|
| Target/Specificity | SIVA1; |
| Reconstitution & Storage | SIVA antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SIVA Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SIVA1 |
|---|---|
| Synonyms | SIVA |
| Function | Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl- x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis. |
| Cellular Location | Cytoplasm. Nucleus. Note=In the nucleus, accumulates in dot- like structures |
| Tissue Location | Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SIVA Antibody: SIVA plays an important role in the apoptotic pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. SIVA is a small cysteine-rich protein with a unique N-terminal amphipathic helical region, a death domain homology region (DDHR) and a C-terminal cysteine-rich B-box-like or zinc-finger-like structure. The DDHR domain binds to CD27 and induces apoptosis in T cells through a caspase-dependent mitochondrial pathway. SIVA inhibits BCL2L1 isoform Bcl-xL anti-apoptotic activity and inhibits activation of NFκ-B and promotes T-cell receptor-mediated apoptosis. SIVA represents a potential therapeutic target for the treatment of neuronal cell death diseases.
References
Prasad KV, Ao Z, Yoon Y, et al. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Natl. Acad. Sci. USA 1997; 94:6346-51.
Py B, Slomianny C, Auberger P, et al. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immunol. 2004; 172:4008-17.
Resch U, Schichl YM, Winsauer G, et al. Siva1 is a XIAP-interacting protein that balances NFkappaB and JNK signalling to promote apoptosis. J. Cell Sci. 2009; 122:2651-61
Jacobs SB, Basak S, Murray JI, et al. Siva is an apoptosis-selective p53 target gene important for neuronal cell death. Cell Death Differ. 2007; 14:1374-85.
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