|Application ||WB, IHC-P, IF, E|
|Other Accession||Q9P2K9, 149274653|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||153048 Da|
|Application Notes||PTCHD2 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PTCHD2; At least two isoforms of PTCHD2 are known to exist. This antibody is specific for PTCHD2 and will not recognize the other DISP family of proteins.|
|Reconstitution & Storage||PTCHD2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PTCHD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in brain and testis.|
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Provided below are standard protocols that you may find useful for product applications.
PTCHD2 Antibody: PTCHD2, also known as DISP3, is the third of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored secreted proteins. Like DISP1 and DISP2, DISP3 has been implicated in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis. It is highly expressed in Purkinje cells, hippocampal neurons, and retinal ganglion cells. Recently, it has been shown that PTCHD2 localizes within the endoplasmic reticulum and colocalizes with cholesterol, and given that its expression is regulated by thyroid hormone (T3), it has been suggested that DISP3 may be a link between thyroid hormone and cholesterol metabolism.
Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6
Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther. 2005; 4:1050-4
Zikova M, Corlett A, Bendova Z, et al. DISP3, a sterol-sensing domain-containing protein that links thyroid hormone action and cholesterol metabolism. Mol. Endocrin. 2009; 23:520-8.
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