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JMJD3 Antibody

     
  • WB - JMJD3 Antibody ASC11429
    Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • ICC - JMJD3 Antibody ASC11429
    Immunocytochemistry of JMJD3 in K562 cells with JMJD3 antibody at 2.5 µg/mL.
  • IF - JMJD3 Antibody ASC11429
    Immunofluorescence of JMJD3 in K562 cells with JMJD3 antibody at 20 µg/mL.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E
Primary Accession O15054
Other Accession EAW90126, 122937251
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 176632 Da
Application Notes JMJD3 antibody can be used for detection of EPAC1 by Western blot at 0.5 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 5 µg/mL.
Additional Information
Gene ID 23135
Target/Specificity KDM6B; This antibody is specific for JMJD3 and will not recognize other JMJD proteins.
Reconstitution & Storage JMJD3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsJMJD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM6B
Synonyms JMJD3, KIAA0346
Function Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402).
Cellular Location Nucleus.
Citations (0)

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Background

JMJD3 Antibody: The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.

References

Agger K, Cloos PA, Christensen J, et al. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 2007; 449:731-4.
De Santa F, Totaro MG, Prosperini E, et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated silencing. Cell 2007; 130:1083-94
Sola S, Xavier JM, Santos DM, et al. p53 interaction with JMJD3 results in its nuclear distribution during mouse neural stem cell differentiation. PLoS One 2011; 6:e18421.

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$ 320.00
Cat# ASC11429
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