|Application ||WB, IF, E|
|Other Accession||NP_002456, 338968860|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||60595 Da|
|Application Notes||SLAIN1 antibody can be used for detection of SLAIN1 by Western blot at 1 µg/mL. Antibody can also be used for immunofluorescence starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SLAIN1; At least three isoforms of SLAIN1 are known to exist; this antibody will detect all three. SLAIN1 antibody is predicted not to cross-react with SLAIN2.|
|Reconstitution & Storage||SLAIN1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SLAIN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Expressed in embryonic stem cells.|
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Provided below are standard protocols that you may find useful for product applications.
SLAIN1 Antibody: SLAIN1 is a novel stem cell protein that was identified by transciptional profiling of mouse and human embryonic stem cells (ESCs) and is expressed at the stem cell and epiblast stages. Unlike its more widely expressed homolog SLAIN2, SLAIN1 was found to be expressed in the epiblast, nervous system, tailbud and somites of the developing mouse embryo later experiments suggested that SLAIN1 may play a role in the development of the nervous system as well as in the morphogenesis of several embryonic structures.
Hirst CE, Ng ES, Azzola L, et al. Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene. Dev. Biol. 2006; 293:90-103.
Hirst CE, Lim SM, Pereira LA, et al. Expression from a betageo gene trap in the Slain1 gene locus is predominantly associated with the developing nervous system. Int. J. Dev. Biol. 2010; 54:1383-8.
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