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NKX2-1 Antibody

  • WB - NKX2-1 Antibody ASC11445
    Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 µg/mL.
  • IHC - NKX2-1 Antibody ASC11445
    Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 µg/mL.
  • IF - NKX2-1 Antibody ASC11445
    Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43699
Other Accession P43699, 118766339
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 38596 Da
Application Notes NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 7080
Target/Specificity NKX2-1; NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
Reconstitution & Storage NKX2-1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNKX2-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX2-1 (HGNC:11825)
Function Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P50220}.
Tissue Location Thyroid and lung.
Citations (0)

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NKX2-1 Antibody: NKX2-1 (NK2 homeobox 1) has been identified as a thyroid-specific transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype and morphogenesis. It contains one homeobox DNA-binding domain. NKX2-1 also may play a role in lung development and surfactant homeostasis. Mutations and deletions in this gene are associated with benign hereditary chorea, congenital hypothyroidism, neonatal respiratory distress, and may be associated with thyroid cancer. NKX2-1 has been suggested to be a candidate suppressor of malignant progression.


Lazzaro D, Price M, Felice MD, et al. The transcription factor TTF-1 is expressed at the onset of thyroid and lung morphogenesis and in restricted regions of the fetal brain. Development 1991; 113:1093-1104.
Guazzi S, Price M, De Felice M, et al. Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity. EMBO J. 1990; 9:3631-9.
Breedveld GJ, Van Dongen JWF et al. Mutations in TITF-1 are associated with benign hereditary chorea. Hum. Mol. Genet. 2002; 11:9719.
Kondo T, Nakazawa T, Ma D, et al. Epigenetic silencing of TTF-1/NKX2-1 through DNA hypermethylation and histone H3 modulation in thyroid carcinomas. Lab Invest. 2009; 89:791-9.

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$ 310.00
Cat# ASC11445
(40 western blots)
Availability: 5-7days
Bulk Size
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