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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   NKX2-2 Antibody   

NKX2-2 Antibody

  • WB - NKX2-2 Antibody ASC11446
    Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - NKX2-2 Antibody ASC11446
    Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 µg/mL.
  • IF - NKX2-2 Antibody ASC11446
    Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95096
Other Accession NP_002500, 4505401
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 30133 Da
Application Notes NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 4821
Target/Specificity NKX2-2; NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
Reconstitution & Storage NKX2-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNKX2-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX2-2
Synonyms NKX2.2, NKX2B
Function Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Research Areas
Citations (0)

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NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.


Sussel L, Kalamaras J, Hartigan-O’Connor DJ, et al. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 1998; 125:2213-21
Prado CL, Pugh-Bernard AE, Elghazi L, et al. Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc. Natl. Acad. Sci. 2004; 101:2924-9.
Hill JT, Chao CS, Anderson KR, et al. Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol. Endocrinol. 2010; 381-90.
Briscoe J, Sussel L, Serup P, et al. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signaling. Nature 1999; 398:622-7.

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$ 310.00
Cat# ASC11446
(40 western blots)
Availability: 5-7days
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