|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001129743, 343183350|
|Calculated MW||32121 Da|
|Application Notes||NKX2-6 antibody can be used for detection of NKX2-6 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL.|
|Target/Specificity||NKX2-6; NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms|
|Reconstitution & Storage||NKX2-6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||NKX2-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional activator (PubMed:15649947). In conjunction with NKX2-5, may play a role in both pharyngeal and cardiac embryonic development.|
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Provided below are standard protocols that you may find useful for product applications.
NKX2-6 Antibody: NKX2-6 (NK2 homeobox 6) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-6 is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are thought to be a cause for some congenital heart abnormalities.
Copeland NG, Jenkins NA, and Harvey RP. The murine homeobox genes Nkx2.3 and Nkx2.6 are located on chromosomes 19 and 14, respectively. Genomics 1994; 22:655-6.
Tanaka M, Kasahara H, Bartunkova S, et al. Vertebrate homologs of tinman and bagpipe: roles of the homeobox genes in cardiovascular development. Dev. Genet. 1998; 22:239-49.
Biben C, Hatzistavrou T, and Harvey RP. Expression of NK-2 class homeobox gene Nkx2-6 in foregut endoderm and heart. Mech. Dev. 1998; 73:125-7
Tanaka M, Schinke M, Liao HS, et al. Nkx2.5 and Nkx2.6, homologs of Drosophila tinman, are required for development of the pharynx. Mol. Cell. Biol. 2001; 21:4391-8.
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