|Application ||WB, IHC-P, E|
|Other Accession||NP_998767, 284266|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||35653 Da|
|Application Notes||SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.|
|Target/Specificity||SIGLEC15 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15.|
The immunogen is located within amino acids 50 - 100 of SIGLEC15.
|Reconstitution & Storage||SIGLEC15 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SIGLEC15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds sialylated glycoproteins.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in macrophage and/or dendritic cells of spleen and lymph nodes|
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Provided below are standard protocols that you may find useful for product applications.
SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Crocker PR and Redelinghuys P. Siglecs as positive and negative regulators of the immune system. Biochem. Soc. Trans. 2008; 36:1467-71.
Angata T, Tabuchi Y, Nakamura K, et al. Siglec-15: an immune system Siglec conserved throughout vertebrate evolution. Glycobiology 2007; 17:838-46
Hiruma Y, Hirai T, and Tsuda E. Siglec-15, a member of the sialic acid-binding lectin, is a novel regulator for osteoclast differentiation. Biochem. Biophys. Res. Commun. 2011; 409:424-9.
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