|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_065758, 239582769|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||56703 Da|
|Application Notes||HHATL antibody can be used for detection of HHATL by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Reconstitution & Storage||HHATL antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||HHATL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C3orf3, GUP1, KIAA1173|
|Function||Negatively regulates N-terminal palmitoylation of SHH by HHAT/SKN.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.
Abe Y, Kita Y, and Niikura T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-palmitoylation of Sonic hedgehog. FEBS J. 2008; 275:318-31.
Zhang SQ, Tian X, Luo YW, et al. Expression, clinical and pathological significance of KIAA1173 gene in skin squamous cell carcinoma. Zhonghua Yi Xue Za Zhi 2010; 90:1243-6.
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