GZF1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9H116 |
Other Accession | NP_071927, 11968150 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 80492 Da |
Application Notes | GZF1 antibody can be used for detection of GZF1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL. |
Gene ID | 64412 |
---|---|
Target/Specificity | GZF1; GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist. |
Reconstitution & Storage | GZF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | GZF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GZF1 (HGNC:15808) |
---|---|
Synonyms | ZBTB23, ZNF336 |
Function | Transcriptional repressor that binds the GZF1 responsive element (GRE) (consensus: 5'-TGCGCN[TG][CA]TATA-3'). May be regulating VSX2/HOX10 expression. |
Cellular Location | Cytoplasm. Nucleus, nucleoplasm Nucleus, nucleolus. Note=Nuclear localization depends upon NCL. |
Tissue Location | Expressed in adult brain, heart, skeletal muscle, kidney and liver. Also detected in fetal brain and kidney, and at lower levels in fetal lung and liver. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
GZF1 Antibody: The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.
References
Fukuda N, Ichihara M, Morinaga T, et al. Identification of a novel glial cell line-derived neurotrophic factor-inducible gene required for renal branching morphogenesis. J. Biol. Chem. 2003; 278:50386-92
Morinaga T, Enomoto A, Shimono Y, et al. GDNF-inducible zinc finger protein 1 is a sequence-specific transcriptional repressor that binds to the HOXA10 gene regulatory region. Nuc. Acids Res. 2005; 33:4191-201.
Dambara A, Morinaga T, Fukuda N, et al. Nucleolin modulates the subcellular localization of GDNF-inducible zinc finger protein 1 and its roles in transcription and cell proliferation. Exp. Cell Res. 2007; 313:3755-66.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.