|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_071927, 11968150|
|Calculated MW||80492 Da|
|Application Notes||GZF1 antibody can be used for detection of GZF1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Target/Specificity||GZF1; GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.|
|Reconstitution & Storage||GZF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||GZF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor. Binds the GZF1 responsive element (GRE) (consensus: 5'-TGCGCN[TG][CA]TATA-3'). May be regulating VSX2/HOX10 expression.|
|Cellular Location||Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Nuclear localization depends upon NCL|
|Tissue Location||Expressed in adult brain, heart, skeletal muscle, kidney and liver. Also detected in fetal brain and kidney, and at lower levels in fetal lung and liver|
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Provided below are standard protocols that you may find useful for product applications.
GZF1 Antibody: The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.
Fukuda N, Ichihara M, Morinaga T, et al. Identification of a novel glial cell line-derived neurotrophic factor-inducible gene required for renal branching morphogenesis. J. Biol. Chem. 2003; 278:50386-92
Morinaga T, Enomoto A, Shimono Y, et al. GDNF-inducible zinc finger protein 1 is a sequence-specific transcriptional repressor that binds to the HOXA10 gene regulatory region. Nuc. Acids Res. 2005; 33:4191-201.
Dambara A, Morinaga T, Fukuda N, et al. Nucleolin modulates the subcellular localization of GDNF-inducible zinc finger protein 1 and its roles in transcription and cell proliferation. Exp. Cell Res. 2007; 313:3755-66.
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