|Application ||IHC-P, IF, E|
|Other Accession||NP_009093, 24307933|
|Calculated MW||N/A KDa|
|Application Notes||TFEB antibody can be used for detection of TFEB by immunohistochemistry at 2.5 µg/ml.|
|Target/Specificity||TFEB; TFEB antibody is human specific. At least three isoforms of TFEB are known to exist.|
|Reconstitution & Storage||TFEB antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||TFEB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.|
|Cellular Location||Cytoplasm. Nucleus Note=Mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). In macrophages, translocates into the nucleus upon live S.enterica infection (PubMed:27184844).|
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Provided below are standard protocols that you may find useful for product applications.
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors (1,2). Recent studies have shown that lysosomal biogenesis is regulated by TFEB (3), which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1 (4). Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes (5).
Carr CS and Sharp PA. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol. Cell Biol. 1990; 10:4384-8.
Davis IJ, Hsi BL, Arroyo JD, et al. Cloning of an alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. Proc. Natl. Acad. Sci. USA 2003; 100:6051-6.
Sardiello M, Palmieri M, di Ronza A, et al. A gene network regulating lysosomal biogenesis and function. Science 2009; 325:473-7.
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases my mTORC1. EMBO J. 2011; 30:3242-58.
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