|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055368, 7657299|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||54627 Da|
|Application Notes||TFCP2L1 antibody can be used for detection of TFCP2L1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Reconstitution & Storage||TFCP2L1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TFCP2L1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional suppressor. May suppress UBP1-mediated transcriptional activation. Required for normal duct development in the salivary gland and kidney (By similarity). Modulates the placental expression of CYP11A1.|
|Tissue Location||Highly expressed in placental JEG-3 cells and very low levels of expression in non-steroidogenic cells. No expression was seen in adrenal NCI-H295A cells or in adrenal tissue.|
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Provided below are standard protocols that you may find useful for product applications.
TFCP2L1 Antibody: The Transcription factor CP2-like protein 1 (TFCP2L1) is related to the ubiquitously expressed CP2 family of transcription factors that are generally transcription factors. TFCP2L1 is expressed in a developmentally regulated fashion during embryogenesis and in the epithelial lining of distal convoluted tubules in embryonic and adult kidneys. Recent studies have shown that TFCP2L1 is part of an Oct4-centered protein interaction network in embryonic stem cells. It is thought to modulate the activity of other CP2 family members in a cell specific manner, and is itself regulated by sumoylation at a single major site.
Rodda S, Sharma S, Scherer M, et al. CRTR-1, a developmentally regulated transcriptional repressor related to the CP2 family of transcription factors. J. Biol. Chem. 2001; 276:3324-32.
van den Berg DL, Snoek T, Mullin NP, et al. An Oct4-centered protein interaction network in embryonic stem cells. Cell Stem Cell 2010; 6:369-81.
To S, Rodda SJ, Rathjen PD, et al. Modulation of CP family transcriptional activity by CRTR-1 and sumoylation. PLoS One 2011; 5:e11702.
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