|Application ||WB, IHC-P, E|
|Other Accession||NP_077005, 56243583|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47405 Da|
|Application Notes||WDR18 antibody can be used for detection of WDR18 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Target/Specificity||WDR18; WDR18 antibody is predicted to not cross-react with other WDR family members.|
|Reconstitution & Storage||WDR18 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||WDR18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role during development (By similarity). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.|
|Cellular Location||Nucleus, nucleoplasm. Cytoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions.|
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Provided below are standard protocols that you may find useful for product applications.
WDR18 Antibody: WD repeat domain 18 (WDR18) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeats are minimally conserved regions of approximately 40 amino acids that commonly form a tertiary propeller structure. WDR18 is a 432 amino acid protein that contains six WD repeats and is encoded by a gene located on human chromosome 19. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit.
Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.
Gao W, Xu L, Guan R, et al. Wdr18 is required for Kupffer's vesicle formation and regulation of body asymmetry in zebrafish. PLoS One 2011; 6:e23386.
Finkbeiner E, Haindl M, and Muller S. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. EMBO J. 2011; 30:1067-78.
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