|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001185951, 312222737|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||113424 Da|
|Application Notes||CCP110 antibody can be used for detection of CCP110 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 5 µg/mL.|
|Target/Specificity||CCP110; Two isoforms of CCP110 are known to exist; this antibody will only detect the longer isoform|
|Reconstitution & Storage||CCP110 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CCP110 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CEP110, CP110, KIAA0419|
|Function||Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Recruited early and then associates with the growing distal tips. Recruited to the mother centriole by KIF24. Removed from centrioles by TTBK2, leading to initiation of ciliogenesis|
|Tissue Location||Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
CCP110 Antibody: CCP110 is a centrosomal protein required for the centrosome to function as a microtubule organizing center and plays an essential role in centrosome duplication. It is a target of cyclin-dependent kinases (CDKs); disruption of CCP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CCP110 is also a target of SCF (Cyclin F) ubiquitin ligase complex, leading to its degradation, suggesting that this complex controls centrosome homeostasis and mitotic fidelity through CCP110 degradation. The centriolar kinesin Kif24 also interacts with CP110 and remodel microtubules and regulate ciliogenesis.
Chen Z, Indjeian VB, McManus M, et al. CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev. Cell 2002; 3:339-50.
D’Angiolella V, Donato V, Vijayakumar S, et al. SCF (Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. Nature 2010; 466:138-42.
Kobayashi T, Tsang WY, Li J, et al. Centriolar kinesin Kif24 interacts with CP110 to remodel microtubules and regulate ciliogenesis. Cell 2011; 145:914-25.
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