|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_620137, 226371723|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||88924 Da|
|Application Notes||FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Target/Specificity||FCHO2; FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist; this antibody will detect only the two longest isoforms.|
|Reconstitution & Storage||FCHO2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||FCHO2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP- 2-dependent endocytosis of the transferin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.|
|Cellular Location||Membrane, clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2 (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
FCHO2 Antibody: FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.
Katoh M and Katoh M. Identification and characterization of human FCHO2 and mouse fcho2 genes in silico. Int. J. Mol. Med. 2004; 14:327-31
Henne WM, Boucrot E, Meinecke M, et al. FCHo proteins are nucleators of clathrin-mediated endocytosis. Science 2010; 328:1281-4.
Uezu A, Umeda K, Tsujita K, et al. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells 2011; 16:868-78.
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