TTYH2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | Q9BSA4 |
Other Accession | NP_116035, 53988385 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 58772 Da |
Application Notes | TTYH2 antibody can be used for detection of TTYH2 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL. |
Gene ID | 94015 |
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Target/Specificity | TTYH2; At least four isoforms of TTYH2 are known to exist; this antibody will only detect the two longest isoforms. This antibody is predicted to not cross-react with other TTYH family members. |
Reconstitution & Storage | TTYH2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | TTYH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TTYH2 |
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Synonyms | C17orf29 |
Function | Probable large-conductance Ca(2+)-activated chloride channel. May play a role in Ca(2+) signal transduction. May be involved in cell proliferation and cell aggregation. |
Cellular Location | Cell membrane; Multi-pass membrane protein |
Tissue Location | Expressed at higher level in brain and testis and at lower levels in heart, ovary, spleen and peripheral blood leukocytes. Up-regulated in 13 of 16 renal cell carcinoma samples examined. Up-regulated in colon carcinoma |
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Provided below are standard protocols that you may find useful for product applications.
Background
TTYH2 Antibody: TTYH2 is a member of the tweety family of proteins, a family of chloride anion channels containing five transmembrane regions. TTYH2 is a Ca2+-activated large conductance chloride (Cl-) channel and may play a role in kidney tumorigenesis. It is also upregulated in colon carcinoma and is thought to play a role in cell proliferation and call aggregation, suggesting that TTYH2 may play a role in regulating both proliferation and metastatic potentials of colorectal cancer. The ubiquitin-protein ligase Nedd4-2 binds to and ubiquitinates TTYH2, thereby regulating its cell surface expression and total cellular levels.
References
Rae FK, Hooper JD, Eyre HJ, et al. TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma. Genomics 2001; 77:200-7.
Toiyama Y, Mizoguchi A, Kimura K, et al. TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation. World J. Gastroenterol. 2007; 13:2717-21
He Y, Hryciw DH, Carroll ML, et al. The ubiquitin-protein ligase Nedd4-2 differentially interacts with and regulates members of the Tweety family of chloride ion channels. J. Biol. Chem. 2008; 283:24000-10.
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