|Application ||WB, IF, ICC, E|
|Other Accession||NP_851853, 31044432|
|Calculated MW||56975 Da|
|Application Notes||LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL.|
|Target/Specificity||LEMD2; At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform.|
|Reconstitution & Storage||LEMD2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||LEMD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in nuclear structure organization (PubMed:16339967). Required for maintaining the integrity of the nuclear envelope (PubMed:17097643).|
|Cellular Location||Nucleus inner membrane; Multi- pass membrane protein. Note=Lamina- associated protein residing in the inner nuclear membrane (INM) Localized exclusively to the nuclear envelope, giving rise to a typical rim-like staining of the nuclear periphery|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.
Brachner A, Reipert S, Foisner R, et al. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 2005; 118:5797-810
Huber MD, Guan T, and Gerace L. Overlapping functions of nuclear envelope proteins NET25 (Lem2) and Emerin in regulation of extracellular signal-regulated kinase signaling in myoblast differentiation. Mol. Cell. Biol. 2009; 29:5718-28.
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