|Application ||WB, IF, E|
|Other Accession||NP_001104505, 161377453|
|Calculated MW||36599 Da|
|Application Notes||TRAP antibody can be used for detection of TRAP by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Reconstitution & Storage||TRAP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TRAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.|
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Provided below are standard protocols that you may find useful for product applications.
TRAP Antibody: TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.
Baumbach GA, Saunders PT, Bazer FW, et al. Uteroferrin has N-apsaragine-linked high mannose-type oligosaccharaides that contain mannose 6-phosphate. Proc. Natl. Acad. Sci. USA 1984; 81:2985-9.
Sun P, Sleat DE, Lecocq M, et al. Acid phosphatase 5 is responsible for removing the mannose 6-phosphate recognition marker from lysosomal proteins. Proc. Natl. Acad. Sci. USA 2004; 105:16590-5.
Makypridi G, Damme M, Muller-Loennies S, et al. Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5. Mol. Cell Biol. 2012; 32:774-82.
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