|Application ||WB, IF, E|
|Other Accession||NP_054727, 7661844|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||70756 Da|
|Application Notes||CCDC22 antibody can be used for detection of CCDC22 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CCDC22; At least three isoforms of CCDC22 are known to exist; this antibody will detect the two largest isoforms.|
|Reconstitution & Storage||CCDC22 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CCDC22 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in regulation of NF-kappa-B signaling. Promotes ubiquitination of I-kappa-B-kinase subunit IKBKB and its subsequent proteasomal degradation leading to NF-kappa-B activation; the function may involve association with COMMD8 and a CUL1-dependent E3 ubiquitin ligase complex. May down-regulate NF- kappa-B activity via assocation with COMMD1 and involving a CUL2- dependent E3 ubiquitin ligase complex. Regulates the cellular localization of COMM domain-containing proteins, such as COMMD1 and COMMD10 (PubMed:23563313). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947).|
|Tissue Location||Widely expressed in adult tissues and in fetal liver and brain, with highest levels in prostate and lowest in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
CCDC22 Antibody: CCDC22 is a recently identified coiled-coil domain-containing protein that has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. In rat, CCDC22 has been observed to localize in multiple regions of the brain, including the prefrontal and somatosensory cortex, dentate gyrus and thalamus, and in the ipsilateral motor neurons of the spinal cord after sciatic nerve transection suggesting that it may play a role in neuronal injury response. The human CCDC22 gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability (XLID).
Tomsig JL, Snyder SL, and Creutz CE. Identification of targets for calcium signaling through the copine family of proteins. J. Biol. Chem. 2003; 278:10048-54.
Mulder J, Bjorling E, Jonasson K, et al. Tissue profiling of the mammalian central nervous system using human antibody-based proteomics. Mol. Cell Prot. 2009; 8:1612-22.
Mulder J, Wernerus H, Shi TJ, et al. Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system. Neurosci.2007; 146:1689-703
Voineagu I, Huang L, Winden K, et al. CCDC22: a novel candidate gene for syndromic X-linked intellectual disability. Mol. Psych. 2012; 17:4-7.
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