|Application ||WB, IF, E|
|Other Accession||NP_001185882, 312147341|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26506 Da|
|Application Notes||ZF21 antibody can be used for detection of ZF21 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ZFYVE21; At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||ZF21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||ZF21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell adhesion, and thereby in cell motility which requires repeated formation and disassembly of focal adhesions. Regulates microtubule-induced PTK2/FAK1 dephosphorylation, an event important for focal adhesion disassembly, as well as integrin beta-1/ITGB1 cell surface expression.|
|Cellular Location||Cell junction, focal adhesion. Cytoplasmic vesicle. Endosome. Note=Within cytoplasmic vesicles, partially colocalizes with EEA1, an endosomal marker|
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Provided below are standard protocols that you may find useful for product applications.
ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
Uekita T, Gotoh I, Kinoshita T, et al. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. 2004; 279:12734-43.
Nagano M, Hoshino D, Sakamoto T, et al. ZF21 protein regulates cell adhesion and motility. J. Biol. Chem. 2010; 285:21013-22.
Nagano M, Hoshino D, Koshiba S, et al. ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain. J. Biol. Chem. 2011; 286:31598-609.
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