|Application ||WB, E|
|Other Accession||NP_006010, 19924145|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||91 kDa|
|Application Notes||TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 µg/mL.|
|Target/Specificity||TCIRG1; At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.|
|Reconstitution & Storage||TCIRG1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||TCIRG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation.|
|Cellular Location||Membrane; Multi-pass membrane protein|
|Tissue Location||Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus|
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Provided below are standard protocols that you may find useful for product applications.
TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic defects in the gene encoding this protein a responsible for a severe form of autosomal recessive osteopetrosis, a condition that leads to increased bone density, decreased bone strength, and inflammation in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.
Toyomura T, Oka T, Yamaguchi C, et al. Three subunit a isoforms of mouse vacuolar H+-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. J. Biol. Chem. 2000; 275:8760-5
Kornak U, Schulz A, Friedrich W, et al. Mutations in the a3 subunit of the vacuolar H+-ATPase cause malignant osteopetrosis. Hum. Mol. Genet. 2000; 9:2059-63.
Sun-Wada GH, Toyomura T, Kawamura N, et al. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic b-cells. J. Cell Sci. 2006; 119:4531-40.
Sun-Wada GH, Tabata H, Kawamura N, et al. Direct recruitment of H+-ATPase from lysosomes for phagocytic acidification. J. Cell Sci. 2009; 122:2504-13.
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