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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NDFIP1 Antibody   

NDFIP1 Antibody

  • WB - NDFIP1 Antibody ASC11525
    Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BT67
Other Accession NP_085048, 13386480
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 24 kDa
Application Notes NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 µg/mL.
Additional Information
Gene ID 80762
Target/Specificity NDFIP1; NDFIP1 antibody is predicted to not cross-react with NDFIP2.
Reconstitution & Storage NDFIP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsNDFIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms N4WBP5
Function Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.
Cellular Location Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Secreted Note=Detected in exosomes and secreted via the exosomal pathway
Tissue Location Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.
Research Areas
Citations (0)

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NDFIP1 Antibody: The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.


Harvey KF, Shearwin-Whyatt LM, Fotia A, et al. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 2002; 277:9307-17.
Sang Q, Kim MH, Kumar S, et al. Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. J. Neurosci. 2006; 26:7234-44.
Mund T and Pelham HR. Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2. Proc. Natl. Acad. Sci. USA 2010; 107; 11429-34.
Ramon HE, Beal AM, Liu Y, et al. The E3 ubiquitin ligase adaptor Ndfip1 regulates Th17 differentiation by limiting the production of proinflammatory cytokines. J. Immunol. 2012; epub.

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$ 310.00
Cat# ASC11525
(40 western blots)
Availability: 5-7days
Bulk Size
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