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CASKIN1 Antibody

     
  • WB - CASKIN1 Antibody ASC11530
    Western blot analysis of CASKIN1 in HeLa cell lysate with CASKIN1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8WXD9
Other Accession NP_065815, 18079216
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 157 kDa
Application Notes CASKIN1 antibody can be used for detection of CASKIN1 by Western blot at 1 µg/mL.
Additional Information
Gene ID 57524
Target/Specificity CASKIN1; CASKIN1 antibody is predicted to not cross-react with other CASKIN family members.
Reconstitution & Storage CASKIN1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCASKIN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASKIN1
Synonyms KIAA1306
Function May link the scaffolding protein CASK to downstream intracellular effectors.
Cellular Location Cytoplasm. EMBL; AF451977; AAL49758.1; -; mRNA EMBL; AB037727; BAA92544.1; -; mRNA CCDS; CCDS42103.1; - RefSeq; NP_065815.1; NM_020764.3 UniGene; Hs.643537; - PDB; 3SEI; X-ray; 2.40 A; A/B=470-605 PDB; 3SEN; X-ray; 3.10 A; A/B/C/D=470-613 PDBsum; 3SEI; - PDBsum; 3SEN; - ProteinModelPortal; Q8WXD9; - SMR; Q8WXD9; - BioGrid; 121585; 3 DIP; DIP-34890N; - IntAct; Q8WXD9; 1 STRING; 9606.ENSP00000345436; - iPTMnet; Q8WXD9; - PhosphoSitePlus; Q8WXD9; - BioMuta; CASKIN1; - DMDM; 61213003; - EPD; Q8WXD9; - MaxQB; Q8WXD9; - PaxDb; Q8WXD9; - PeptideAtlas; Q8WXD9; - PRIDE; Q8WXD9; - ProteomicsDB; 75015; - Ensembl; ENST00000343516; ENSP00000345436; ENSG00000167971 GeneID; 57524; - KEGG; hsa:57524; - UCSC; uc010bsg.2; human CTD; 57524; - EuPathDB; HostDB:ENSG00000167971.15; - GeneCards; CASKIN1; - HGNC; HGNC:20879; CASKIN1 HPA; CAB079050; - HPA; HPA055990; - MIM; 612184; gene neXtProt; NX_Q8WXD9; - OpenTargets; ENSG00000167971; - PharmGKB; PA134878267; - eggNOG; ENOG410INAR; Eukaryota eggNOG; COG0666; LUCA GeneTree; ENSGT00920000148972; - HOGENOM; HOG000049168; - HOVERGEN; HBG051133; - InParanoid; Q8WXD9; - KO; K21952; - OMA; KIQGSPT; - OrthoDB; EOG091G00P3; - PhylomeDB; Q8WXD9; - TreeFam; TF320582; - ChiTaRS; CASKIN1; human GenomeRNAi; 57524; - PRO; PR:Q8WXD9; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000167971; - CleanEx; HS_CASKIN1; - Genevisible; Q8WXD9; HS GO; GO:0005737; C:cytoplasm; ISS:UniProtKB GO; GO:0016020; C:membrane; IBA:GO_Central GO; GO:0042802; F:identical protein binding; IPI:IntAct GO; GO:0007165; P:signal transduction; ISS:UniProtKB CDD; cd00204; ANK; 2 CDD; cd09497; SAM_caskin1_2_repeat1; 1 CDD; cd09498; SAM_caskin1_2_repeat2; 1 CDD; cd12062; SH3_Caskin1; 1 Gene3D; 1.25.40.20; -; 2 InterPro; IPR002110; Ankyrin_rpt InterPro; IPR020683; Ankyrin_rpt-contain_dom InterPro; IPR036770; Ankyrin_rpt-contain_sf InterPro; IPR027013; Caskin-1 InterPro; IPR032232; Caskin1-CID InterPro; IPR035497; Caskin1/2_SAM_1 InterPro; IPR035498; Caskin1/2_SAM_2 InterPro; IPR035495; Caskin1_SH3 InterPro; IPR032117; Caskin_C InterPro; IPR001660; SAM InterPro; IPR013761; SAM/pointed_sf InterPro; IPR036028; SH3-like_dom_sf InterPro; IPR001452; SH3_domain PANTHER; PTHR24177:SF12; PTHR24177:SF12; 1 Pfam; PF12796; Ank_2; 2 Pfam; PF16632; Caskin-tail; 1 Pfam; PF16600; Caskin1-CID; 1 Pfam; PF00536; SAM_1; 2 Pfam; PF07653; SH3_2; 1 PRINTS; PR01415; ANKYRIN SMART; SM00248; ANK; 6 SMART; SM00454; SAM; 2 SMART; SM00326; SH3; 1 SUPFAM; SSF47769; SSF47769; 2 SUPFAM; SSF48403; SSF48403; 1 SUPFAM; SSF50044; SSF50044; 1 PROSITE; PS50297; ANK_REP_REGION; 1 PROSITE; PS50088; ANK_REPEAT; 6 PROSITE; PS50105; SAM_DOMAIN; 2 PROSITE; PS50002; SH3; 1 1: Evidence at protein level; 3D-structure; ANK repeat; Complete proteome; Cytoplasm; Methylation; Phosphoprotein; Reference proteome; Repeat; SH3 domain CHAIN 1 1431 Caskin-1 /FTId=PRO_0000066980 REPEAT 48 77 ANK 1 REPEAT 81 110 ANK 2 REPEAT 114 143 ANK 3 REPEAT 147 176 ANK 4 REPEAT 188 217 ANK 5 REPEAT 220 249 ANK 6 DOMAIN 281 347 SH3. {ECO:0000255|PROSITE- ProRule:PRU00192} DOMAIN 472 535 SAM 1. {ECO:0000255|PROSITE- ProRule:PRU00184} DOMAIN 541 605 SAM 2. {ECO:0000255|PROSITE- ProRule:PRU00184} REGION 375 469 CASK-binding. COMPBIAS 714 1365 Pro-rich MOD_RES 253 253 Phosphotyrosine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 358 358 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 398 398 Omega-N-methylarginine MOD_RES 423 423 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 432 432 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 633 633 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 646 646 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 719 719 Phosphoserine {ECO:0000250|UniProtKB:Q8VHK2} MOD_RES 724 724 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 737 737 Phosphothreonine {ECO:0000250|UniProtKB:Q8VHK2} MOD_RES 787 787 Phosphoserine MOD_RES 889 889 Phosphoserine {ECO:0000250|UniProtKB:Q8VHK2} MOD_RES 891 891 Phosphoserine {ECO:0000250|UniProtKB:Q8VHK2} MOD_RES 987 987 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 1065 1065 Phosphothreonine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 1067 1067 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 1257 1257 Phosphoserine MOD_RES 1266 1266 Phosphothreonine {ECO:0000250|UniProtKB:Q6P9K8} MOD_RES 1364 1364 Phosphoserine {ECO:0000250|UniProtKB:Q6P9K8} CONFLICT 278 278 R -> P (in Ref. 2; BAA92544) HELIX 470 482 {ECO:0000244|PDB:3SEI} TURN 483 485 {ECO:0000244|PDB:3SEI} HELIX 487 489 {ECO:0000244|PDB:3SEI} HELIX 490 495 {ECO:0000244|PDB:3SEI} HELIX 500 503 {ECO:0000244|PDB:3SEI} HELIX 508 513 {ECO:0000244|PDB:3SEI} HELIX 519 530 {ECO:0000244|PDB:3SEI} HELIX 546 552 {ECO:0000244|PDB:3SEI} HELIX 556 558 {ECO:0000244|PDB:3SEI} HELIX 559 564 {ECO:0000244|PDB:3SEI} HELIX 570 573 {ECO:0000244|PDB:3SEI} HELIX 578 584 {ECO:0000244|PDB:3SEI} HELIX 589 605 {ECO:0000244|PDB:3SEI} SEQUENCE 1431 AA; 149814 MW; CDF60E68B81E884A CRC64; MGKEQELVQA VKAEDVGTAQ RLLQRPRPGK AKLLGSTKKI NVNFQDPDGF SALHHAALNG NTELISLLLE AQAAVDIKDN KGMRPLHYAA WQGRKEPMKL VLKAGSAVNI PSDEGHIPLH LAAQHGHYDV SEMLLQHQSN PCMVDNSGKT PLDLACEFGR VGVVQLLLSS NMCAALLEPR PGDATDPNGT SPLHLAAKNG HIDIIRLLLQ AGIDINRQTK SGTALHEAAL CGKTEVVRLL LDSGINAHVR NTYSQTALDI VHQFTTSQAS REIKQLLREA SAALQVRATK DYCNNYDLTS LNVKAGDIIT VLEQHPDGRW KGCIHDNRTG NDRVGYFPSS LGEAIVKRAG SRAGTEPSLP QGSSSSGPSA PPEEIWVLRK PFAGGDRSGS ISGMAGGRGS GGHALHAGSE GVKLLATVLS QKSVSESGPG DSPAKPPEGS AGVARSQPPV AHAGQVYGEQ PPKKLEPASE GKSSEAVSQW LTAFQLQLYA PNFISAGYDL PTISRMTPED LTAIGVTKPG HRKKIAAEIS GLSIPDWLPE HKPANLAVWL SMIGLAQYYK VLVDNGYENI DFITDITWED LQEIGITKLG HQKKLMLAVR KLAELQKAEY AKYEGGPLRR KAPQSLEVMA IESPPPPEPT PADCQSPKMT TFQDSELSDE LQAAMTGPAE VGPTTEKPSS HLPPTPRATT RQDSSLGGRA RHMSSSQELL GDGPPGPSSP MSRSQEYLLD EGPAPGTPPR EARPGRHGHS IKRASVPPVP GKPRQVLPPG TSHFTPPQTP TKTRPGSPQA LGGPHGPAPA TAKVKPTPQL LPPTERPMSP RSLPQSPTHR GFAYVLPQPV EGEVGPAAPG PAPPPVPTAV PTLCLPPEAD AEPGRPKKRA HSLNRYAASD SEPERDELLV PAAAGPYATV QRRVGRSHSV RAPAGADKNV NRSQSFAVRP RKKGPPPPPP KRSSSALASA NLADEPVPDA EPEDGLLGVR AQCRRASDLA GSVDTGSAGS VKSIAAMLEL SSIGGGGRAA RRPPEGHPTP RPASPEPGRV ATVLASVKHK EAIGPGGEVV NRRRTLSGPV TGLLATARRG PGESADPGPF VEDGTGRQRP RGPSKGEAGV EGPPLAKVEA SATLKRRIRA KQNQQENVKF ILTESDTVKR RPKAKEREAG PEPPPPLSVY HNGTGTVRRR PASEQAGPPE LPPPPPPAEP PPTDLAHLPP LPPPEGEARK PAKPPVSPKP VLTQPVPKLQ GSPTPTSKKV PLPGPGSPEV KRAHGTPPPV SPKPPPPPTA PKPVKAVAGL PSGSAGPSPA PSPARQPPAA LAKPPGTPPS LGASPAKPPS PGAPALHVPA KPPRAAAAAA AAAAAPPAPP EGASPGDSAR QKLEETSACL AAALQAVEEK IRQEDAQGPR DSAAEKSTGS ILDDIGSMFD DLADQLDAML E
Citations (0)

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Background

CASKIN1 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting proteins) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN1 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. CASKIN1 coassembles with CASK on the cytoplasmic tail of neurexin 1, suggesting that CASKIN and CASK coat the cytoplasmic tails of neurexins and other cell-surface proteins.

References

Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
Weng YL, Liu N, DiAntonio A, et al. The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance. Neurosci. 2011; 31:4421-33.
Tabuchi K, Biederer T, Butz S, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with CASKIN1, a novel CASK-binding protein. J. Neurosci. 2002; 22:4264-73.

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Cat# ASC11530
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