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BCAR3 Antibody

     
  • WB - BCAR3 Antibody ASC11531
    Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.
  • IF - BCAR3 Antibody ASC11531
    Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O75815
Other Accession NP_003558, 4502371
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 91 kDa
Application Notes BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 8412
Target/Specificity BCAR3; At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
Reconstitution & Storage BCAR3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsBCAR3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCAR3
Synonyms NSP2, SH2D3B
Function May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Tissue Location Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue EMBL; U92715; AAC39777.1; -; mRNA EMBL; AF124250; AAD28245.1; -; mRNA EMBL; AY358996; AAQ89355.1; -; mRNA EMBL; AL833121; -; NOT_ANNOTATED_CDS; mRNA EMBL; AL049796; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL109613; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL359820; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL512488; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471097; EAW73065.1; -; Genomic_DNA EMBL; CH471097; EAW73066.1; -; Genomic_DNA EMBL; CH471097; EAW73068.1; -; Genomic_DNA EMBL; BC039895; AAH39895.1; -; mRNA CCDS; CCDS58010.1; -. [O75815-3] CCDS; CCDS745.1; -. [O75815-1] CCDS; CCDS76181.1; -. [O75815-2] RefSeq; NP_001248337.1; NM_001261408.1. [O75815-1] RefSeq; NP_001248338.1; NM_001261409.1. [O75815-1] RefSeq; NP_001248339.1; NM_001261410.1. [O75815-3] RefSeq; NP_001295180.1; NM_001308251.1. [O75815-2] RefSeq; NP_003558.1; NM_003567.3. [O75815-1] RefSeq; XP_016857969.1; XM_017002480.1. [O75815-1] UniGene; Hs.36958; - PDB; 3T6A; X-ray; 2.40 A; A/B/C/D=502-825 PDBsum; 3T6A; - ProteinModelPortal; O75815; - SMR; O75815; - BioGrid; 114000; 38 DIP; DIP-33857N; - IntAct; O75815; 77 MINT; O75815; - STRING; 9606.ENSP00000260502; - iPTMnet; O75815; - PhosphoSitePlus; O75815; - BioMuta; BCAR3; - EPD; O75815; - MaxQB; O75815; - PaxDb; O75815; - PeptideAtlas; O75815; - PRIDE; O75815; - ProteomicsDB; 50205; - ProteomicsDB; 50206; -. [O75815-2] DNASU; 8412; - Ensembl; ENST00000260502; ENSP00000260502; ENSG00000137936. [O75815-1] Ensembl; ENST00000370243; ENSP00000359263; ENSG00000137936. [O75815-1] Ensembl; ENST00000370244; ENSP00000359264; ENSG00000137936. [O75815-1] Ensembl; ENST00000370247; ENSP00000359267; ENSG00000137936. [O75815-3] Ensembl; ENST00000539242; ENSP00000441343; ENSG00000137936. [O75815-2] GeneID; 8412; - KEGG; hsa:8412; - UCSC; uc001dpx.6; human. [O75815-1] CTD; 8412; - DisGeNET; 8412; - EuPathDB; HostDB:ENSG00000137936.16; - GeneCards; BCAR3; - HGNC; HGNC:973; BCAR3 HPA; HPA014858; - MIM; 604704; gene neXtProt; NX_O75815; - OpenTargets; ENSG00000137936; - PharmGKB; PA25283; - eggNOG; ENOG410IFQG; Eukaryota eggNOG; ENOG410XTJR; LUCA GeneTree; ENSGT00390000008976; - HOGENOM; HOG000231595; - HOVERGEN; HBG053174; - InParanoid; O75815; - OMA; NYCELNP; - OrthoDB; EOG091G01KG; - PhylomeDB; O75815; - TreeFam; TF323756; - SignaLink; O75815; - ChiTaRS; BCAR3; human GeneWiki; BCAR3; - GenomeRNAi; 8412; - PRO; PR:O75815; - Proteomes; UP000005640; Chromosome 1 Bgee; ENSG00000137936; - CleanEx; HS_BCAR3; - Genevisible; O75815; HS GO; GO:0005622; C:intracellular; IEA:GOC GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW GO; GO:0005070; F:SH3/SH2 adaptor activity; IBA:GO_Central GO; GO:0002089; P:lens morphogenesis in camera-type eye; IEA:Ensembl GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IEA:Ensembl GO; GO:0042493; P:response to drug; TAS:ProtInc GO; GO:0007165; P:signal transduction; TAS:ProtInc GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro CDD; cd00155; RasGEF; 1 Gene3D; 1.10.840.10; -; 1 Gene3D; 3.30.505.10; -; 1 InterPro; IPR028849; BCAR3 InterPro; IPR023578; Ras_GEF_dom_sf InterPro; IPR001895; RASGEF_cat_dom InterPro; IPR036964; RASGEF_cat_dom_sf InterPro; IPR000980; SH2 InterPro; IPR036860; SH2_dom_sf PANTHER; PTHR14247:SF10; PTHR14247:SF10; 1 Pfam; PF00617; RasGEF; 1 Pfam; PF00017; SH2; 1 SMART; SM00147; RasGEF; 1 SMART; SM00252; SH2; 1 SUPFAM; SSF48366; SSF48366; 1 SUPFAM; SSF55550; SSF55550; 1 PROSITE; PS50009; RASGEF_CAT; 1 PROSITE; PS50001; SH2; 1 1: Evidence at protein level; 3D-structure; Acetylation; Alternative splicing; Complete proteome; Guanine-nucleotide releasing factor; Methylation; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain INIT_MET 1 1 Removed. CHAIN 2 825 Breast cancer anti-estrogen resistance protein 3 /FTId=PRO_0000230284 DOMAIN 154 253 SH2. {ECO:0000255|PROSITE- ProRule:PRU00191} DOMAIN 548 818 Ras-GEF. {ECO:0000255|PROSITE- ProRule:PRU00168} COMPBIAS 479 482 Poly-Asp MOD_RES 2 2 N-acetylalanine MOD_RES 32 32 Phosphoserine MOD_RES 78 78 Phosphoserine MOD_RES 83 83 Phosphoserine MOD_RES 182 182 Phosphoserine MOD_RES 290 290 Phosphoserine MOD_RES 334 334 N6-methyllysine MOD_RES 358 358 Phosphoserine MOD_RES 363 363 Phosphoserine MOD_RES 375 375 Phosphoserine MOD_RES 442 442 Omega-N-methylarginine MOD_RES 471 471 Phosphoserine {ECO:0000250|UniProtKB:Q9QZK2} VAR_SEQ 1 324 Missing (in isoform 2) /FTId=VSP_017814 VAR_SEQ 1 91 Missing (in isoform 3) /FTId=VSP_046716 VAR_SEQ 92 117 ESPWQDRHGETFTFRDPHLLDPTVEY -> MPKECSAFHAL SAALCCFYHRKSFIG (in isoform 3) /FTId=VSP_046717 VARIANT 464 464 E -> G (in dbSNP:rs12062278) /FTId=VAR_050689 VARIANT 593 593 Q -> H (in dbSNP:rs17110107) /FTId=VAR_050690 CONFLICT 519 519 E -> G (in Ref. 4; AL833121) CONFLICT 543 543 L -> P (in Ref. 4; AL833121) HELIX 517 519 {ECO:0000244|PDB:3T6A} HELIX 534 546 {ECO:0000244|PDB:3T6A} HELIX 549 563 {ECO:0000244|PDB:3T6A} HELIX 571 577 {ECO:0000244|PDB:3T6A} STRAND 578 580 {ECO:0000244|PDB:3T6A} HELIX 582 585 {ECO:0000244|PDB:3T6A} HELIX 589 591 {ECO:0000244|PDB:3T6A} HELIX 592 614 {ECO:0000244|PDB:3T6A} HELIX 620 639 {ECO:0000244|PDB:3T6A} HELIX 644 655 {ECO:0000244|PDB:3T6A} HELIX 657 660 {ECO:0000244|PDB:3T6A} HELIX 663 672 {ECO:0000244|PDB:3T6A} HELIX 674 682 {ECO:0000244|PDB:3T6A} HELIX 684 692 {ECO:0000244|PDB:3T6A} HELIX 696 698 {ECO:0000244|PDB:3T6A} HELIX 711 717 {ECO:0000244|PDB:3T6A} HELIX 724 726 {ECO:0000244|PDB:3T6A} HELIX 732 734 {ECO:0000244|PDB:3T6A} HELIX 737 752 {ECO:0000244|PDB:3T6A} HELIX 754 764 {ECO:0000244|PDB:3T6A} TURN 765 767 {ECO:0000244|PDB:3T6A} HELIX 772 777 {ECO:0000244|PDB:3T6A} HELIX 780 792 {ECO:0000244|PDB:3T6A} HELIX 797 815 {ECO:0000244|PDB:3T6A} SEQUENCE 825 AA; 92566 MW; C55D61BB4AC7D0F2 CRC64; MAAGKFASLP RNMPVNHQFP LASSMDLLSS RSPLAEHRPD AYQDVSIHGT LPRKKKGPPP IRSCDDFSHM GTLPHSKSPR QNSPVTQDGI QESPWQDRHG ETFTFRDPHL LDPTVEYVKF SKERHIMDRT PEKLKKELEE ELLLSSEDLR SHAWYHGRIP RQVSENLVQR DGDFLVRDSL SSPGNFVLTC QWKNLAQHFK INRTVLRLSE AYSRVQYQFE MESFDSIPGL VRCYVGNRRP ISQQSGAIIF QPINRTVPLR CLEEHYGTSP GQAREGSLTK GRPDVAKRLS LTMGGVQARE QNLPRGNLLR NKEKSGSQPA CLDHMQDRRA LSLKAHQSES YLPIGCKLPP QSSGVDTSPC PNSPVFRTGS EPALSPAVVR RVSSDARAGE ALRGSDSQLC PKPPPKPCKV PFLKVPSSPS AWLNSEANYC ELNPAFATGC GRGAKLPSCA QGSHTELLTA KQNEAPGPRN SGVNYLILDD DDRERPWEPA AAQMEKGQWD KGEFVTPLLE TVSSFRPNEF ESKFLPPENK PLETAMLKRA KELFTNNDPK VIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIE RHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSAL MKALEMPQIT RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCV PPNNVSVPLL MPLVTLMERQ AVTFEGTDMW EKNDQSCEIM LNHLATARFM AEAADSYRMN AERILAGFQP DEEMNEICKT EFQMRLLWGS KGAQVNQTER YEKFNQILTA LSRKLEPPPV KQAEL
Citations (0)

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Background

BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.

References

van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.
Riggins RB, Quilliam LA, and Bouton AH. Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3. J. Biol. Chem. 2003; 278:28264-73.
Schuh NR, Guerrero MS, Schrecengost RS, et al. BCAR3 regulates Src/p130Cas association, Src kinase activity, and breast cancer adhesion signaling. J. Biol. Chem. 2010; 285:2309-17.

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Cat# ASC11531
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