|Application ||WB, IF, E|
|Other Accession||NP_003558, 4502371|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||91 kDa|
|Application Notes||BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||BCAR3; At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.|
|Reconstitution & Storage||BCAR3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||BCAR3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.|
|Tissue Location||Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue|
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Provided below are standard protocols that you may find useful for product applications.
BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.
van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.
Riggins RB, Quilliam LA, and Bouton AH. Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3. J. Biol. Chem. 2003; 278:28264-73.
Schuh NR, Guerrero MS, Schrecengost RS, et al. BCAR3 regulates Src/p130Cas association, Src kinase activity, and breast cancer adhesion signaling. J. Biol. Chem. 2010; 285:2309-17.
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