|Application ||WB, IF, E|
|Other Accession||NP_056483, 158534077|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||139 kDa|
|Application Notes||RGS22 antibody can be used for detection of RGS22 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||RGS22; At least four isoforms of RGS22 are known to exist; this antibody will detect the three longest isoforms. RGS22 antibody is predicted to not cross-react with other RGS proteins|
|Reconstitution & Storage||RGS22 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||RGS22 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.|
|Cellular Location||Cytoplasm. Nucleus. Note=Expressed in the cytoplasm of spermatogonia and spermatocytes. In spermatids, also expressed in the nucleus|
|Tissue Location||Testis-specific. Expressed in Leydig cells and spermatogenic cells from the spermatogonia to spermatid stages (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
RGS22 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS22 is a recently identified member of this family that localizes to the testis and can interact with guanine nucleotide binding proteins alpha 11, 12, and 13 (GNA11, GNA12, and GNA13). While RGS22 has been postulated to play a role in spermiogenesis in the testis, it is also expressed in several cancer cell lines with an epithelial origin and associated with cancer metastasis. Its overexpression in a highly metastatic cancer causes a decrease in cell migration and a reduction of the invasive potential of the cells, suggesting that RGS22 may be a potential prognostic biomarker for metastasis.
De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA 1995; 92:11916-20.
Berman DM, Wilkie TM, and Gilman AG. GAIP and RGS4 are GTP-ase activating proteins for the Gi subfamily of G protein alpha subunits. Cell 1996; 86:445-52.
Hu Y, Xing J, Chen L, et al. RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol. Reprod. 2008; 79:1021-9
Hu Y, Xing J, Wang L, et al. RGS22, a novel cancer/testis antigen, inhibits epithelial cell invasion and metastasis. Clin. Exp. Metastasis 2011; 28:541-9.
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