|Application ||WB, IF, E|
|Other Accession||NP_003788, 24041020|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49 kDa|
|Application Notes||EED antibody can be used for detection of EED by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||EED; Four alternatively spliced transcript variants have been observed.|
|Reconstitution & Storage||EED antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||EED Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.|
|Cellular Location||Nucleus. Chromosome. Note=Transiently colocalizes with XIST at inactive X chromosomes|
|Tissue Location||Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer|
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Provided below are standard protocols that you may find useful for product applications.
EED Antibody: The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.
Rietzler M, Bittner M, Kolanus W, et al. The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins. J. Biol. Chem. 1998; 273:27459-66.
Brock HW and Fisher CL. Maintenance of gene expression patterns. Dev Dyn. 2005; 232:633-55.
Levine SS, King IF, Kingston RE, et al. Division of labor in polycomb group repression. Trends Biochem. Sci. 2004; 29: 478-85.
Van der Vlag J and Otte AP. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat. Genet.1999; 23:474-8.
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