|Application ||WB, IF, E|
|Other Accession||NP_060600, 56550033|
|Calculated MW||63 kDa|
|Application Notes||SHQ1 antibody can be used for detection of SHQ1 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SHQ1; Multiple isoforms are known to exist.|
|Reconstitution & Storage||SHQ1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SHQ1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the quantitative accumulation of H/ACA ribonucleoproteins (RNPs), including telomerase, probably through the stabilization of DKC1, from the time of its synthesis until its association with NOP10, NHP2, and NAF1 at the nascent H/ACA RNA.|
|Cellular Location||Cytoplasm, cytosol. Nucleus, nucleoplasm. Note=May at least partially shuttle between cytosol and nucleoplasm. In the nucleoplasm, exhibits a granular pattern, but is excluded from nucleoli and Cajal bodies Absent from most H/ACA-box RNA transcription sites|
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Provided below are standard protocols that you may find useful for product applications.
SHQ1 Antibody: SHQ1 is the human homolog of yeast Shq1p, a protein that acts as an accessory factor in the assembly of H/ACA ribonucleoproteins. SHQ1 has been shown to bind the major H/ACA core protein and the pseudouridine synthase NAP57, but precedes the assembly role of NAF1 and of the other H/ACA core proteins. SHQ1 assists in the assembly of H/ACA-box ribonucleoproteins that function in the processing of ribosomal RNAs, modification of spliceosomal small nuclear RNAs, and stabilization of telomerase.
Ito T, Chiba T, Ozawa R, et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc. Natl. Acad. Sci. USA 2001; 98:4569-74.
Grozdanov PN, Roy S, Kittur N, et al. SHQ1 is required prior to NAF1 for assembly of H/ACA small nucleolar and telomerase RNPs. RNA 2009; 15:1188-97.
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