SGSM2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, E |
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Primary Accession | O43147 |
Other Accession | NP_055668, 148612795 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 116 kDa |
Application Notes | SGSM2 antibody can be used for detection of SGSM2 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 9905 |
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Target/Specificity | SGSM2; Multiple isoforms of SGSM2 are known to exist. SGSM2 antibody is predicted to not cross react with other SGSM family proteins. |
Reconstitution & Storage | SGSM2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | SGSM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SGSM2 |
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Synonyms | KIAA0397, RUTBC1 |
Function | Possesses GTPase activator activity towards RAB32, RAB33B and RAB38 (PubMed:26620560, PubMed:21808068). Regulates the trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes by inactivating RAB32 and RAB38. Inhibits RAB32 and RAB38 activation both directly by promoting their GTPase activity and indirectly by disrupting the RAB9A-HPS4 interaction which is required for RAB32/38 activation (PubMed:26620560). |
Cellular Location | Cytoplasm. Melanosome. Note=Melanosomal localization is mediated by RAB9A. |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SGSM2 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. SGSM2 is a Rab9A effector that activates GTP hydrolysis by Rab32 and Rab33B proteins.
References
Bar-Sagi D and Hall A. Ras and Rho GTPases: a family reunion. Cell 2000; 103:227-38.
Colicelli J. Human ras superfamily proteins and related GTPases. Sci. STKE 2004; 250:RE13
Yang H, Sasaki T, Minoshima S, et al. Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway. Genomics 2007; 90:249-60.
Nottingham RM, Ganley IG, Barr FA, et al. RUTBC1 protein, a Rab9A effector that activates GTP hydrolysis by Rab32 and Rab33B proteins. J. Biol. Chem. 2011; 286:33213-22.
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