|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_002648, 5326|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||55 kDa|
|Application Notes||PLAGL2 antibody can be used for detection of PLAGL2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||PLAGL2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human PLAGL2.|
The immunogen is located within amino acids 40 - 90 of PLAGL2.
|Reconstitution & Storage||PLAGL2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||PLAGL2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Shows weak transcriptional activatory activity.|
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Provided below are standard protocols that you may find useful for product applications.
PLAGL2 Antibody: The PLAG (Pleiomorphic adenoma gene) family of zinc finger proteins include PLAG1, ZAC1 and PLAG-like 2 (PLAGL2). PLAG1 is the target gene for the pleiomorphic adenomas of the salivary gland. ZAC1 (PLAGL1) concomitantly controls apoptosis and cell cycle arrest through separate pathways. ZAC1 also acts as a transcriptional cofactor for nuclear receptors. PLAGL2 belongs to the krueppel C2H2-type zinc-finger protein family and contains 6 C2H2-type zinc fingers. It functions as a positive regulator of transcription and localizes to the nucleus. PLAGL2 is ubiquitously expressed with particular abundance in spleen, lung and testis, where it may be involved in cell cycle arrest and apoptosis of tumor cells.
Kas K, Voz ML, Hensen K, et al. Transcriptional activation capacity of the novel PLAG family of zinc finger proteins. J. Biol. Chem. 1998; 273:23026-32.
Voz ML, Agten NS, Van de Ven WJ, et al. PLAG1, the main translocation target in pleomorphic adenoma of the salivary glands, is a positive regulator of IGF-II. Cancer Res. 2000; 60:106-13.
Shu G, Tang Y, Zhou Y, et al. Zac1 is a histone acetylation-regulated NF-kappaB suppressor that mediates histone deacetylase inhibitor-induced apoptosis. Cell Death Differ. 2011; 18:1825-35.
Mizutani A, Furukawa T, Adachi Y, et al. A zinc-finger protein, PLAGL2, induces the expression of a proapoptotic protein Nip3, leading to cellular apoptosis. J. Biol. Chem. 2002; 277:15851-8.
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