|Application ||WB, IHC-P, E|
|Other Accession||Q9NY84, 88952267|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||55 kDa|
|Application Notes||VNN3 antibody can be used for detection of VNN3 by Western blot at 0.5 and 1 µg/mL.|
|Target/Specificity||VNN3; Multiple isoforms of VNN3 are known to exist.|
|Reconstitution & Storage||VNN3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||VNN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor. Note=According to PubMed:11491533, secreted.|
|Tissue Location||Widely expressed with higher expression in liver and blood. Expressed in differentiated keratinocytes in epidermis and in epithelial cells in dermis. Overexpressed in lesional psoriatic skin.|
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Provided below are standard protocols that you may find useful for product applications.
VNN3 Antibody: The vanin family is a novel group of ectoenzymes that function in tissue repair and plays a role in oxidative-stress response. As both secreted and membrane proteins, the vanin family members have been implicated as therapeutic targets in inflammatory disease. VNN3 (vascular non-inflammatory molecule 3), also known as Vanin3, is a 501 amino acid GPI-anchored amidohydrolase that is widely expressed and is found at highest levels in blood and liver. Induced by Th17 / Th1 type cytokines, VNN3 converts pantetheine into pantothenic acid. Containing one CN hydrolase domain, VNN3 is encoded by a gene that maps to human chromosome 6q23.2.
Granjeaud S, Naquet P and Galland F. An ESTs description of the new Vanin gene family conserved from fly to human. Immunogenetics 1999; 49:964-72.
Jansen PA, Kamsteeg M, Rodijk-Olthuis D, et al. Expression of the vanin gene family in normal and inflamed human skin: induction by proinflammatory cytokines. J. Invest. Dermatol. 2009;129:2167-74.
Nitto T, Inoue T and Node K. Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils. Gene 2008; 426:57-64.
Martin F, Malergue F, Pitari G, et al. Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes. Immunogenetics 2001; 53:296-306.
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