Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
CMPK2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q5EBM0 |
| Other Accession | NP_997198, 117606370 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 49 kDa |
| Application Notes | CMPK2 antibody can be used for detection of CMPK2 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 129607 |
|---|---|
| Target/Specificity | CMPK2; Multiple isoforms of CMPK2 are known to exist. |
| Reconstitution & Storage | CMPK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | CMPK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CMPK2 |
|---|---|
| Function | Mitochondrial nucleotide monophosphate kinase needed for salvage dNTP synthesis that mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways (PubMed:17999954, PubMed:30083606, PubMed:36930652, PubMed:37075076). Restricts the replication of multiple viruses including flaviviruses or coronaviruses (PubMed:30083606, PubMed:36930652, PubMed:37075076). Together with viperin/RSAD2 and ddhCTP, suppresses the replication of several coronaviruses through inhibition of the viral RNA-dependent RNA polymerase activities (PubMed:36930652). Concerning flaviviruses, restricts RNA translation when localized to the mitochondria independently of its kinase activity (PubMed:37075076). Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP while CMP and UMP are poor substrates. Controls therefore mitochondrial DNA synthesis by supplying required deoxyribonucleotides (By similarity). CMPK2-dependent mitochondrial DNA synthesis is necessary for the production of oxidized mitochondrial DNA fragments after exposure to NLRP3 activators (By similarity). In turn, cytosolic oxidized mtDNA associates with the NLRP3 inflammasome complex and is required for its activation (By similarity). |
| Cellular Location | Mitochondrion Note=Mitochondrial localization is required for its antiviral function |
| Tissue Location | High levels are observed in myeloid, lymphoid and mesenchymal tissues. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
CMPK2 Antibody: UMP-CMP kinase 2 (CMPK2) is the first nucleoside monophosphate kinase that has been identified in human mitochondria. It is a component of the salvage pathway for nucleotide synthesis that may participate in terminal differentiation of monocytic cells. There are two distinct domains of CMPK2: the N-terminal domain with unknown function and the C-terminal domain with the kinase function, suggesting that CMPK2 may be a bifunctional protein with other biological functions in addition to its UMP-CMP kinase activity. CMPK2 may participate in dUTP and dCTP synthesis and is responsible for phosphorylation of dCMP and dUMP in mitochondria. CMPK2 is actively involved in macrophage activation and the inflammatory response
References
Xu Y, Johansson M, Karlsson A. Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondria. J. Biol. Chem. 2008; 283:1563-71.
Chen YL, Lin DW, and Chang ZF. Identification of a putative human mitochondrial thymidine monophosphate kinase associated with monocytic/macrophage terminal differentiation. Genes Cells 2008; 13:679-89.
Arima T, Akiyoshi H, and Fujii S. Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues. Cancer Res. 1977; 37:1593-7.
Goertsches RH, Hecker M, Koczan D, et al. Long-term genome-wide blood RNA expression profiles yield novel molecular response candidates for IFN-beta-1b treatment in relapsing remitting MS. Pharmacogenomics 2010;11:147-61.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
