|Application ||WB, IF, E|
|Other Accession||NP_997198, 117606370|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49 kDa|
|Application Notes||CMPK2 antibody can be used for detection of CMPK2 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||CMPK2; Multiple isoforms of CMPK2 are known to exist.|
|Reconstitution & Storage||CMPK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||CMPK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. May be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. Also displays broad nucleoside diphosphate kinase activity.|
|Tissue Location||Among all investigated tumors, leukemia cells show the most abundant expression.|
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Provided below are standard protocols that you may find useful for product applications.
CMPK2 Antibody: UMP-CMP kinase 2 (CMPK2) is the first nucleoside monophosphate kinase that has been identified in human mitochondria. It is a component of the salvage pathway for nucleotide synthesis that may participate in terminal differentiation of monocytic cells. There are two distinct domains of CMPK2: the N-terminal domain with unknown function and the C-terminal domain with the kinase function, suggesting that CMPK2 may be a bifunctional protein with other biological functions in addition to its UMP-CMP kinase activity. CMPK2 may participate in dUTP and dCTP synthesis and is responsible for phosphorylation of dCMP and dUMP in mitochondria. CMPK2 is actively involved in macrophage activation and the inflammatory response
Xu Y, Johansson M, Karlsson A. Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondria. J. Biol. Chem. 2008; 283:1563-71.
Chen YL, Lin DW, and Chang ZF. Identification of a putative human mitochondrial thymidine monophosphate kinase associated with monocytic/macrophage terminal differentiation. Genes Cells 2008; 13:679-89.
Arima T, Akiyoshi H, and Fujii S. Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues. Cancer Res. 1977; 37:1593-7.
Goertsches RH, Hecker M, Koczan D, et al. Long-term genome-wide blood RNA expression profiles yield novel molecular response candidates for IFN-beta-1b treatment in relapsing remitting MS. Pharmacogenomics 2010;11:147-61.
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