|Application ||WB, E|
|Other Accession||NP_056425, 7661580|
|Calculated MW||50 kDa|
|Application Notes||WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||WIPI2; WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.|
|Reconstitution & Storage||WIPI2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||WIPI2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.|
|Cellular Location||Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.|
|Tissue Location||Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas Expression is down-regulated in pancreatic and in kidney tumors|
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Provided below are standard protocols that you may find useful for product applications.
WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Smith TF. Diversity of WD-repeat proteins. Subcell. Biochem. 2008; 48:20-30.
Polson HE, de Lartique J, Rigden DJ, et al. Mammalian ATG18 (WIPI2) localizes to moegasome-anchored phagophores and positively regulates LC3 lipidation. Autophagy 2010; 6:506-22.
Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.
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