|Application ||WB, E|
|Other Accession||NP_001034461, 86439957|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||51 kDa|
|Application Notes||HEPACAM2 antibody can be used for detection of HEPACAM2 by Western blot at 0.5 - 1 µg/mL.|
|Target/Specificity||HEPACAM2; HEPACAM2 antibody is human, mouse and rat reactive. At least two isoforms of HEPACAM2 are known to exist; this antibody will detect both isoforms. HEPACAM2 antibody is predicted to not cross-react with HEPACAM.|
|Reconstitution & Storage||HEPACAM2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||HEPACAM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation.|
|Cellular Location||Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody Note=In interphase, localizes to the Golgi apparatus. Localizes to centrosomes and spindles during prophase, prometaphase, and metaphase of mitosis, and to midbodies at telophase. Translocation to mitotic centrosomes is the result of poly-ADP-ribosylation (PARsylation)|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
HEPACAM2 Antibody: HEPACAM2 (Hepatocyte cell adhesion molecule 2), a type I N-linked transmembrane glycoprotein, belongs to the immunoglobulin superfamily. The exact function of HEPACAM2 is currently unknown, but the related protein HEPACAM forms cishomodimers on the cell surface to regulate the cell adhesion and may inhibit cell growth through suppression of cell proliferation. HEPACAM and HEPACAM2 mRNA are differentially regulated in canine tumors, with HEPACAM2 mRNA showing increased levels in adenomas, but decreased levels in metastatic carcinomas compared to normal tissues, while HEPACAM protein levels decreased in adenomas. It is therefore likely that HEPACAM2 plays a different role than HEPACAM in the development and progression to tumors.
Klopfleisch R, Klose P, da Costa A, et al. HEPACAM1 and 2 are differentially regulated in canine mammary adenomas and carcinomas and its lymph nodes metastases. BMC Veterinary Res. 2010; 6:15.
Moh MC, Tian Q, Zhang T, et al. The immunoglobulin-like cell adhesion molecule HEPACAM modulate cell adhesion and motility through direct interaction with the actin cytoskeleton. J. Cell Physiol. 2009; 219:382-91.
Lee LH, Moh MC, Zhang T, et al. The immunoglobulin-like cell adhesion molecule HEPACAM induces differentiation of human glioblastoma U373-MG cells. J. Cell Biochem. 2009; 107:1129-38.
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