|Application ||WB, E|
|Other Accession||NP_001166171, 289577098|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||94 kDa|
|Application Notes||SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||SHROOM1; SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members.|
|Reconstitution & Storage||SHROOM1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.|
|Precautions||SHROOM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the assembly of microtubule arrays during cell elongation.|
|Cellular Location||Cytoplasm, cytoskeleton.|
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Provided below are standard protocols that you may find useful for product applications.
SHROOM1 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.
Lee C, Le MP, and Wallingford JB. The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets. Dev. Dyn. 2009; 238:1480-91.
Hildebrand JD and Soriano P. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell 1999; 99:486-97
Hildebrand JD. Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network. J. Cell Sci. 2005; 118:5191-203.
Sevilla-Perez J, Konigshoff M, Kwapiszewska G, et al. Shroom expression is attenuated in pulmonary arterial hypertension. Eur. Respir. J. 2008; 32:871-880.
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