|Application ||WB, E|
|Other Accession||NP_115719, 14150134|
|Calculated MW||Predicted: 26 kDa|
|Application Notes||CHCHD6 antibody can be used for detection of CHCHD6 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||CHCHD6; CHCHD6 is predicted to not cross-react to other members of the CHCHD family.|
|Reconstitution & Storage||CHCHD6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CHCHD6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.|
|Cellular Location||Mitochondrion inner membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
CHCHD6 Antibody: CHCHD6 is a member of a family of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain and has been observed in a complex with the mitochondrial proteins mitofilin, SAM50, metaxins 1 and 2, and CHCHD3. CHCHD6 knockdown causes severe defects in mitochondrial cristae morphology as well as reductions in cell growth, ATP production and oxygen consumption. This decrease of CHCHD6 also led to a reduction in mitofilin protein levels, while a knockdown of mitofilin resulted in a reduced level of CHCHD6, suggesting coordinate regulation between these two proteins. CHCHD6 knockdown in human cancer cells enhances their sensitivity to genotoxic anticancer drugs, suggesting that CHCHD6 may be a potential therapeutic anti-tumor target.
Xie J, Marusich MF, Souda P, et al. The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS Lett. 2007; 581:3545-9.
An J, Shi J, He Q, et al. CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. J. Biol. Chem. 2012; 287:7411-26.
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