|Application ||WB, E|
|Other Accession||NP_057095, 67078404|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 41 kDa|
|Application Notes||MECR antibody can be used for detection of MECR by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||MECR; At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||MECR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||MECR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2- decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis.|
|Cellular Location||Isoform 1: Mitochondrion|
|Tissue Location||Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas Weakly or not expressed in lung.|
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Provided below are standard protocols that you may find useful for product applications.
MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33.
Chen Z, Leskinen H, Liimatta E, et al. Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse. PLoS One 2009; 4:e5589.
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