|Application ||WB, E|
|Other Accession||NP_001180569, 302370926|
|Calculated MW||Predicted: 142 kDa|
|Application Notes||CRB1 antibody can be used for detection of CRB1 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||CRB1; Alternatively spliced transcript variants have been observed. CRB1 antibody i spredicted to not cross-react with CRB2.|
|Reconstitution & Storage||CRB1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CRB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in photoreceptor morphogenesis in the retina. May maintain cell polarization and adhesion.|
|Cellular Location||Isoform 1: Apical cell membrane; Single-pass type I membrane protein. Note=Distributed at the apical membrane of all retinal epithelial cells. Located in the apical membrane of the adherens junction in outer limiting membrane (OLM) of the retina|
|Tissue Location||Preferential expression in retina, also expressed in brain, testis, fetal brain and fetal eye|
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Provided below are standard protocols that you may find useful for product applications.
CRB1 Antibody: CRB1 (Crumbs homolog 1) plays a role in photoreceptor morphogenesis in the retina. It may maintain cell polarization and adhesion. CRB1 is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. The first identified human homolog, CRB1, is expressed in retina and some parts of the brain, leaving room for another homolog to function in epithelial tissues. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis.
Van de Pavert SA, Kantardzhieva A, Malysheva A, et al. Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure. J. Cell Sci. 2004; 117:4169-77.
Van de Pavert SA, Sanz AS, Aartsen WM, et al. Crb1 is a determinant of retinal apical Muller glia cell features. Glia 2007; 55:1486-97.
Den Hollander AI, Johnson K, de Kok YJ, et al. CRB1 has a cytoplasmic domain that is functionally conserved between human and Drosophila. Hum. Mol. Genet. 2001; 10:2767-73.
Bujakowska K, Audo I, Mohand-Said S, et al. CRB1 mutations in inherited retinal dystrophies. Hum. Mutat. 2012; 33:306-15.
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