|Application ||WB, E|
|Other Accession||NP_775960, 112420992|
|Calculated MW||Predicted: 141 kDa|
|Application Notes||CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||CRB2; At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.|
|Reconstitution & Storage||CRB2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CRB2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in polarized cells morphogenesis.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in retina, fetal eye and brain. Also expressed in kidney, ARPE-19 and RPE/choroid cell lines, and at low levels in lung, placenta, and heart|
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Provided below are standard protocols that you may find useful for product applications.
CRB2 Antibody: CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.
van den Hurk J, Rashbass P, Roepman R, et al. Characterization of the Crumbs homolog 2 (CRB2) gene and analysis of its role in retinits pigmentosa and Leber congenital amaurosis. Mol. Viso. 2005; 11:263-73
Bulgakova NA and Knust E. The Crumbs complex: from epithelial-cell polarity to retinal degeneration. J. Cell Sci. 2009; 122:2587-96.
Boroviak T and Rashbass P. The apical polarity determinant Crumbs 2 is a novel regulator of ESC-derived neural progenitors. Stem Cells 2011; 29:193-205
Bujakowska K, Audo I, Mohand-Said S, et al. CRB1 mutations in inherited retinal dystrophies. Hum. Mutat. 2012; 33:306-15
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