|Application ||WB, IHC-P, E|
|Other Accession||NP_065916, 167830433|
|Calculated MW||Predicted: 111 kDa|
|Application Notes||DHX36 antibody can be used for detection of DHX36 by Western blot at 0.5 - 1 µg/mL.|
|Target/Specificity||DHX36; At least four isoforms of DDX36 are known to exist; this antibody will detect the three longest isoforms.|
|Reconstitution & Storage||DHX36 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DHX36 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DDX36, KIAA1488, MLEL1, RHAU|
|Function||Proposed to have a global role in regulating mRNA expression including transcriptional regulation and mRNA stability. Binds with high affinity to and resolves tetramolecular RNA and DNA quadruplex structures. Unwinds intramolecular quadruplexes derived from the ZIC1 and the MYC promoters. Binds to quadruplex structures in the promoters of YY1 and ALPL genes and regulates their expression. Binds to telomerase RNA template component (TERC) 5'-end (nucleotides 1-43) and unwinds an internal quadruplex formation in TERC 5'-end to promote P1 helix formation; the P1 helix acts as a template boundary ensuring accurate reverse transcription and is disrupted by quadruplex formation. May be involved in regulation of telomere length. Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. May play a role in ossification.|
|Cellular Location||Nucleus. Cytoplasm. Chromosome, telomere. Note=Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. Localizes to cytoplasmic stress granules|
|Tissue Location||Highly expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
DHX36 Antibody: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. DHX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE (RHAU), belongs to RNA helicase of the DEAH family and may function in sex development and spermatogenesis. It is expressed in testis and is evolutionary conserved with true orthologs in almost all animal species. DHX36 plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. DHX36 is required for early embryogenesis.
Cordin O, Banroques J, Tanner NK, et al. The DEAD-box protein family of RNA helicases. Gene 2006; 367:17-37.
Linder P. Dead-box proteins: a family affair—active and passive players in RNP-remodeling. Nucleic Acids Res. 2006; 34:4168-80.
Lattmann S, Giri B, Vaughn JP, et al. Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU. Nucleic Acids Res. 2010; 38:6219-33.
Fu JJ, Li LY, Liu SF, et al. Expression research for human DDX36 and mouse Ddx36 gene in the adult testis. Yi Chuan Xue Bao 2003; 30: 201-208.
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