|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001231, 17978466|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 80 kDa|
|Application Notes||CCNT1 antibody can be used for detection of CCNT1 by Western blot at 1 - 2 µg/mL.|
|Reconstitution & Storage||CCNT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CCNT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
CCNT1 Antibody: Cyclins function as regulators of CDK kinases and exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin-T1 protein (CCNT1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNT1 tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing CCNT1 and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. CCNT1 and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.
Uhlmann F, Bouchoux C, and Lopez-Aviles S. A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited. Philos. Trans. R. Soc. Lond. B. biol. Sci. 2011; 366:3572-83.
Wei P, Garber ME, Fang SM, et al. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell 1998; 92:451-62.
Mancebo HS, Lee G, Flygare J, et al. P-TEFb kinase is require for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev. 1997 ; 11 :2633-44.
Majello B and Napolitano G. Control of RNA polymerase II activity by dedicated CTD kinases and phosphatases. Front. Biosci. 2001; 6:D1358-68.
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