|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_002975, 4506845|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 10 kDa|
|Application Notes||CCL4 antibody can be used for detection of CCL4 by Western blot at 1 - 2 µg/mL.|
|Reconstitution & Storage||CCL4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CCL4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||LAG1, MIP1B, SCYA4|
|Function||Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CCL4 Antibody: CCL4, also known as macrophage inflammatory protein 1-beta (MIP1B), belongs to the intercrine beta (chemokine CC) family. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ; with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells.
Baixeras E, Roman-Roman S, Jitsukawa S, et al. Cloning and expression of a lymphocyte activation gene (LAG-1). Mol. Immunol. 1990; 27:1091-102.
Maurer M and von Stebut E. Macrophage inflammatory protein-1. Int. J. Biochem. Cell Biol. 2004; 36:1882-6.
Margolis LB, Glushakova S, Grivel JC, et al. Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines. J. Clin. Invest. 1998; 101:1876-80.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.