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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PIBF1 Antibody   

PIBF1 Antibody

     
  • WB - PIBF1 Antibody ASC11618
    Western blot analysis of PIBF1 in human placenta tissue lysate with PIBF1 antibody at (A) 1 and (B) 2 µg/mL
  • IHC - PIBF1 Antibody ASC11618
    Immunohistochemistry of PBIF in spleen tissue with PBIF antibody at 5 µg/ml.
  • IF - PIBF1 Antibody ASC11618
    Immunofluorescence of PBIF in human spleen tissue with PBIF antibody at 20 µg/ml.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q8WXW3
Other Accession NP_006337, 5576958
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 83 kDa
Application Notes PIBF1 antibody can be used for detection of PIBF1 by Western blot at 1 - 2 µg/mL.
Additional Information
Gene ID 10464
Target/Specificity PIBF1; Multiple isoforms of PIBF1 exists as a result of alternative splicing event.
Reconstitution & Storage PIBF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsPIBF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIBF1
Synonyms C13orf24, PIBF
Function Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed:21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed:23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed:26297806).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm. Secreted. Note=In progesterone-treated astrocytoma cells a 57 kDa protein and isoform 1 (90 kDa) have been described, both being located in the intracellular medium and secreted. Respective predominant forms are isoform 1 in the intracellular and the 57 kDa protein in the extracellular medium (PubMed:25218441). Isoform 4: Secreted Note=Secreted by progesterone-treated lymphocytes (PubMed:14634107).
Tissue Location Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon (PubMed:11935316). Expressed in the first trimester pregnancy decidua (PubMed:12516630). Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms (PubMed:18817979). Overexpresed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer (PubMed:25218441)
Research Areas
Citations (0)

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Background

PIBF1 Antibody: PIBF1 is synthesized during pregnancy in response to progesterone by T lymphocytes. PIBF1 inhibits arachidonic acid release, controls NK activity, and modifies the cytokine balance exerting an anti-abortive effect. It contains a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an ER membrane retention signal and N-glycosylation and phosphorylation sites. PIBF1 is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF1 is associated with the nucleus and functions as a transcription factor, whereas secretion of shorter forms which may act as cytokines is induced by activation of the cell.

References

Laskarin G, Tokmadzic VS, Strbo N, et al. Progesterone induced blocking factor (PIBF) mediates progesterone induced suppression of decidual lymphocyte cytotoxicity. Am. J. Reprod. Immunol. 2002; 48:201-9.
Lachmann M, Gelbmann D, Kalman E, et al. PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome. Int. J. Cancer. 2004; 112:51-60
Polgar B, Kispal G, Lachmann M, et al. Molecular cloning and immunologic characterization of a novel cDNA coding for progesterone-induced blocking factor. J. Immunol. 2003; 171:5956-63.
Raghupathy R, Al-Mutawa E, Al-Azemi M, et al. Progesterone-induced blocking factor (PIBF) modulates cytokine production by lymphocytes from women with recurrent miscarriage or preterm delivery. J. Reprod. Immunol. 2009; 80:91-9.

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$ 320.00
Cat# ASC11618
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