|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_036364, 42734430|
|Calculated MW||43 kDa|
|Application Notes||PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 µg/mL.|
|Target/Specificity||PTRF; At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family.|
|Reconstitution & Storage||PTRF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PTRF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.|
|Cellular Location||Membrane, caveola. Cell membrane Microsome. Endoplasmic reticulum. Cytoplasm, cytosol. Mitochondrion. Nucleus Note=Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co- localizes with CAV1 in lipid rafts in adipocytes.|
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Provided below are standard protocols that you may find useful for product applications.
PTRF Antibody: The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
Jansa P, Mason SW, Hoffmann-Rohrer U, et al. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 1998; 17:2855-64.
Vinten J, Johnsen AH, Roepstorff P, et al. Identification of a major protein on the cytosolic face of caveolae. Biochim. Biophys. Acta 2005; 1717:34-40.
Aboulaich N, Ortegren U, Vener AV, et al. Association and insulin regulated translocation of hormone-sensitive lipase with PTRF. Biochem. Biophys. Res. Commun. 2006; 350:657-61.
Aboulaich N, Chui PC, Asara JM, et al. Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. Diabetes 2011; 60:757-65.
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