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Nucleobindin-2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P80303 |
| Other Accession | NP_005004, 4826870 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 46 kDa Observed: 54 kDa |
| Application Notes | Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 µg/mL. |
| Gene ID | 4925 |
|---|---|
| Target/Specificity | NUCB2; Nucleobindin-2 antibody is predicted to not cross-react with NUCB1. |
| Reconstitution & Storage | Nucleobindin-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | Nucleobindin-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NUCB2 (HGNC:8044) |
|---|---|
| Synonyms | NEFA |
| Function | Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity). |
| Cellular Location | Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm Secreted. Endoplasmic reticulum. Nucleus envelope. Note=Golgi retention is mediated by its N-terminal region |
| Tissue Location | Predominantly expressed in spleen, testis and normal stomach. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
References
Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85.
Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443:709-12.
Garcia-Galiano D, Navarro VM, Gaytan F, et al. Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J. Mol. Endocrin. 2010; 45:281-90.
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