|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_005004, 4826870|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 46 kDa |
Observed: 54 kDa
|Application Notes||Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 µg/mL.|
|Target/Specificity||NUCB2; Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.|
|Reconstitution & Storage||Nucleobindin-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||Nucleobindin-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-binding protein. May have a role in calcium homeostasis.|
|Cellular Location||Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted. Endoplasmic reticulum. Nucleus envelope. Note=Golgi retention is mediated by its N-terminal region|
|Tissue Location||Predominantly expressed in spleen, testis and normal stomach.|
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Provided below are standard protocols that you may find useful for product applications.
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85.
Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443:709-12.
Garcia-Galiano D, Navarro VM, Gaytan F, et al. Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J. Mol. Endocrin. 2010; 45:281-90.
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