|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_057712, 18379328|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 71 kDa |
Observed: 75 kDa
|Application Notes||WAC Antibody can be used for detection of WAC by Western blot at 1 µg/mL.|
|Target/Specificity||WAC; At least two isoforms of WAC are known to exist; this antibody will detect the larger isoform.|
|Reconstitution & Storage||WAC antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||WAC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. May negatively regulate the ubiquitin proteasome pathway.|
|Cellular Location||Nucleus speckle. Nucleus. Note=In distinct nuclear speckles Colocalizes with pre-mRNA processing complexes (By similarity)|
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Provided below are standard protocols that you may find useful for product applications.
WAC Antibody: The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.
Xu GM and Arnaout MA. WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35. Genomics 2002; 79:87-94.
Zhang F and Yu X. WAC, a functional partner of RNF20/40, regulates histone H2B ubiquitination and gene transcription. Mol. Cell 2011; 41:384-97.
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