|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001008389, 56605994|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 15 kDa |
Observed: 15 kDa
|Application Notes||CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.|
|Reconstitution & Storage||CISD2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||CISD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CDGSH2, ERIS, ZCD2|
|Function||Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca(2+) stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion outer membrane; Single-pass membrane protein. Note=According to PubMed:20010695, it mainly localizes to the endoplasmic reticulum. However, experiments in mouse showed that it mainly localizes to the mitochondrion outer membrane|
|Tissue Location||Testis, small intestine, kidney, lung, brain, heart, pancreas and platelets.|
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Provided below are standard protocols that you may find useful for product applications.
CISD2 Antibody: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.
Amr S, Heisey C, Zhang M, et al. A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2. Am. J. Hum. Genet. 2007; 81:673-83.
Chang NC, Nguyen M, Germain M, et al. Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1. EMBO J. 2010; 29:606-18.
Chen YF, Kao CH, Chen YT, et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice.
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