|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055973, 64464726|
|Calculated MW||Predicted: 149 kDa |
Observed: 150 kDa
|Application Notes||KANK1 Antibody can be used for detection of KANK1 by Western blot at 1 µg/mL.|
|Target/Specificity||KANK1; Two alternatively spliced transcript variants encoding different isoforms have been identified. The lower molecular weight band seen in the immunoblot is thought to be non-specific.|
|Reconstitution & Storage||KANK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||KANK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ANKRD15, KANK, KIAA0172|
|Function||Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma.|
|Cellular Location||Cell projection, ruffle membrane Note=Colocalizes with KIF21A in membrane ruffles Isoform 2: Cytoplasm. Nucleus. Cell projection, ruffle membrane. Note=Shuttles between the cytoplasm and nucleus|
|Tissue Location||Widely expressed. Isoform 1 is predominantly expressed in heart and kidney. Isoform 2 probably is widely expressed at basic levels.|
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Provided below are standard protocols that you may find useful for product applications.
KANK1 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK1 (KN motif and ankyrin repeat domain-containing protein 1), also known as ANKRD15, is a 1,352 amino acid protein that contains at least 12 exons and 5 ANK repeats. It binds to beta-catenin and regulates its subcellular distribution. KANK1 is ubiquitously expressed and localizes to cytoplasm. It may function as a tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder.
Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
Sarkar S, Roy BC, Hatano N, et al. A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma. J. Biol. Chem. 2002; 277:36585-91.
Lerer I, Sagi M, Meiner V, et al. Deletion of the ANKRD15 gene at 9p24.3 causes parent-of-origin-dependent inheritance of familial cerebral palsy. Hum. Mol. Genet. 2005; 14: 3911-20
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