|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_056308, 258613875|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 95 kDa |
Observed: 102 kDa
|Application Notes||KANK2 Antibody can be used for detection of KANK2 by Western blot at 1 µg/mL.|
|Target/Specificity||KANK2; Two alternatively spliced transcript variants encoding different isoforms have been identified.|
|Reconstitution & Storage||KANK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||KANK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ANKRD25, KIAA1518, MXRA3, SIP|
|Function||Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. Involved in the negative control of vitamin D receptor signaling pathway (PubMed:24671081). May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus (PubMed:22371500). May be involved in promotion of cell proliferation (By similarity).|
|Cellular Location||Cytoplasm. Mitochondrion.|
|Tissue Location||Strongly expressed in cervix, colon, heart, kidney and lung. Expressed in kidney glomerular podocytes and mesangial cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
KANK2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK2 (KN motif and ankyrin repeat domain-containing protein 2), was initially identified as a steroid receptor coactivator (SRC) interacting protein (SIP) that could sequester steroid receptor coactivators in the cytoplasm. More recent experiments have shown that while KANK2 is widely expressed, in kidney podocytes, KANK2 localizes to foot processes, suggesting that KANK2 may contribute to controlling actin dynamics in podocyte foot processes.
Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
Zhang Y, Zhang H, Liang J, et al. SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm. EMBO J. 2007; 26:2645-57.
Xu X, Patrakka J, Sistani L, et al. Expression of novel podocyte-associated proteins sult1b1 and ankrd25. Nephron Exp. Nephrol. 2011; 117:e39-46.
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