|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_443174, 16418425|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 64 kDa |
Observed: 66 kDa
|Application Notes||GBP5 Antibody can be used for detection of GBP5 by Western blot at 1 µg/mL.|
|Target/Specificity||GBP5; GBP5 antibody is predicted to not cross-react with other GBP family members.|
|Reconstitution & Storage||GBP5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||GBP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||As an activator of NLRP3 inflammasome assembly, plays a role in innate immunity and inflammation. Promotes selective NLRP3 inflammasome assembly in response to microbial and soluble, but not crystalline, agents (PubMed:22461501). Hydrolyzes GTP, but in contrast to other family members, does not produce GMP (PubMed:20180847).|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Lipid- anchor|
|Tissue Location||Expressed in peripheral blood monocytes (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
GBP5 Antibody: The guanylate-binding proteins (GBPs) are a family of interferon-induced GTP-binding proteins function in innate immunity against microbial and viral pathogens. In humans, there are seven GBPs from hGBP1 to hGBP7. Human GBP1 and GBP2 have been shown to exhibit antiviral activity as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to interferon (IFN). GBP5 promotes selective NALP3 inflammasome responses to pathogenic bacteria and soluble but not crystalline inflammasome priming agents.
Vestal DJ. The guanylate-binding proteins (GBPs): proinflammatory cytokine-induced members of the dynamin superfamily with unique GTPase activity. J. Interferon Cytokine Res. 2005; 25:435-43.
Kresse A, Konermann C, Degrandi D, et al. Analyses of murine GBP homology clusters based on in silico, in vitro and in vivo studies. BMC Genomics 2008; 9:158.
Hammon M, Herrmann M, Bleiziffer O, et al. Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases. J. Cell Mol. Med. 2011; 15:1582-92.
Shenoy AR, Wellington DA, Kumar P, et al. GBP5 promotes NLRP3 inflammasome assembly and immunity in mammals. Science 2012; 336:481-5.
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